The light responsivity of ortho-nitrobenzyl esters (o-NBE) is exploited to inscribe mu-scale 2.5D patterns in thiol-ene networks by direct laser writing. For this purpose, a multifunctional thiol and a photosensitive alkene with an o-NBE chromophore are cured upon visible light exposure without inducing a premature photocleavage of the o-NBE links. Once the network is formed, a laser beam source with a wavelength of 375 nm is used for selectively inducing the photocleavage reaction of the o-NBE groups. Positive tone patterns are directly inscribed onto the sample surface without the requirement of a subsequent development step (removing soluble species in an appropriate organic solvent). Along with the realization of dry-developable micropatterns, the chemical surface composition of the exposed areas can be conveniently adjusted since different domains with a tailored content of carboxylic groups are obtained simply by modulating the laser energy dose. In a following step, those are activated and exploited as anchor points for attaching an Alexa-546 conjugated Protein A. Thus, the laser writable thiol-ene networks do not only provide a convenient method for the fabrication of positive tone patterns but also open future prospectives for a wide range of biosensing applications.

Laser-Triggered Writing and Biofunctionalization of Thiol-Ene Networks / Romano, A.; Angelini, A.; Rossegger, E.; Palmara, G.; Castellino, M.; Frascella, F.; Chiappone, A.; Chiad(`o), A.; Sangermano, M.; Schl(\,. - In: MACROMOLECULAR RAPID COMMUNICATIONS. - ISSN 1022-1336. - 41:10(2020), p. e2000084. [10.1002/marc.202000084]

Laser-Triggered Writing and Biofunctionalization of Thiol-Ene Networks

Angelini, A.;
2020

Abstract

The light responsivity of ortho-nitrobenzyl esters (o-NBE) is exploited to inscribe mu-scale 2.5D patterns in thiol-ene networks by direct laser writing. For this purpose, a multifunctional thiol and a photosensitive alkene with an o-NBE chromophore are cured upon visible light exposure without inducing a premature photocleavage of the o-NBE links. Once the network is formed, a laser beam source with a wavelength of 375 nm is used for selectively inducing the photocleavage reaction of the o-NBE groups. Positive tone patterns are directly inscribed onto the sample surface without the requirement of a subsequent development step (removing soluble species in an appropriate organic solvent). Along with the realization of dry-developable micropatterns, the chemical surface composition of the exposed areas can be conveniently adjusted since different domains with a tailored content of carboxylic groups are obtained simply by modulating the laser energy dose. In a following step, those are activated and exploited as anchor points for attaching an Alexa-546 conjugated Protein A. Thus, the laser writable thiol-ene networks do not only provide a convenient method for the fabrication of positive tone patterns but also open future prospectives for a wide range of biosensing applications.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11696/63070
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