The ability to control cell alignment represents a fundamental requirement toward the production of tissue in vitro but also to create biohybrid materials presenting the functional properties of human organs. However, cell cultures on standard commercial supports do not provide a selective control on the cell organization morphology, and different techniques, such as the use of patterned or stimulated substrates, are developed to induce cellular alignment. In this work, a new approach toward in vitro muscular tissue morphogenesis is presented exploiting liquid crystalline networks. By using smooth polymeric films with planar homogeneous alignment, a certain degree of cellular order is observed in myoblast cultures with direction of higher cell alignment corresponding to the nematic director. The molecular organization inside the polymer determines such effects since no cell organization is observed using homeotropic or isotropic samples. These findings represent the first example of cellular alignment induced by the interaction with a nematic polymeric scaffold, setting the stage for new applications of liquid crystal polymers as active matter to control tissue growth.

Liquid Crystal-Induced Myoblast Alignment / Martella, Daniele; Pattelli, Lorenzo; Matassini, Camilla; Ridi, Francesca; Bonini, Massimo; Paoli, Paolo; Baglioni, Piero; Wiersma, Diederik S; Parmeggiani, Camilla. - In: ADVANCED HEALTHCARE MATERIALS. - ISSN 2192-2659. - 8:3(2019), p. e1801489. [10.1002/adhm.201801489]

Liquid Crystal-Induced Myoblast Alignment

Martella, Daniele;Pattelli, Lorenzo;Wiersma, Diederik S;
2019

Abstract

The ability to control cell alignment represents a fundamental requirement toward the production of tissue in vitro but also to create biohybrid materials presenting the functional properties of human organs. However, cell cultures on standard commercial supports do not provide a selective control on the cell organization morphology, and different techniques, such as the use of patterned or stimulated substrates, are developed to induce cellular alignment. In this work, a new approach toward in vitro muscular tissue morphogenesis is presented exploiting liquid crystalline networks. By using smooth polymeric films with planar homogeneous alignment, a certain degree of cellular order is observed in myoblast cultures with direction of higher cell alignment corresponding to the nematic director. The molecular organization inside the polymer determines such effects since no cell organization is observed using homeotropic or isotropic samples. These findings represent the first example of cellular alignment induced by the interaction with a nematic polymeric scaffold, setting the stage for new applications of liquid crystal polymers as active matter to control tissue growth.
File in questo prodotto:
File Dimensione Formato  
adhm201801489_postprint_main.pdf

accesso aperto

Descrizione: post-peer reviewed manuscript version for self-archiving purposes
Tipologia: Documento in Post-print
Licenza: Pubblico - Tutti i diritti riservati
Dimensione 1.16 MB
Formato Adobe PDF
1.16 MB Adobe PDF Visualizza/Apri
adhm.201801489.pdf

non disponibili

Tipologia: Versione editoriale
Licenza: Non Pubblico - Accesso privato/ristretto
Dimensione 3.78 MB
Formato Adobe PDF
3.78 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
adhm.201970009.pdf

non disponibili

Descrizione: journal frontispiece
Tipologia: Altro materiale allegato
Licenza: Non Pubblico - Accesso privato/ristretto
Dimensione 4.09 MB
Formato Adobe PDF
4.09 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11696/61193
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 30
  • ???jsp.display-item.citation.isi??? 32
social impact