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EnglishFluorescence lifetime detection is widely used for sensing physical and chemical quantities. The measurement accuracy of fluorescence lifetime-based sensing systems, either in time or frequency domain relies on their capability of detection and analysis of low level signal superimposed to noise. In this work a quantitative assessment of several data processing and analysis methods for the estimation of the fluorescence lifetime was carried out by using an experimental arrangement based on a fiber optic temperature sensor system. A comparison between the various methods was performed using actual signals from an optical sensing medium. The basic principles of time- and frequency-domain lifetime measurements were also reviewed and discussed in order to point out the limit of the cw frequency-domain approach and to suggest a way to overcome it. The investigated lifetime interval was from 200 to about 2200 s, corresponding to a temperature span of the sensor of about 300 °C. The results showed that in time domain such as with Marquardt, integration, and log-fit algorithms a good agreement, with relative differences from 0.2% to 0.5%, can be reached. Frequency-domain results based on an N-point fast Fourier transform FFT compare favorably with the previous ones in the long lifetime region resulting in relative differences lower than 0.2% with larger differences for short lifetimes. For each data processing method, the uncertainty associated with lifetime estimation was evaluated. Sampling and harmonics effects on the estimation accuracy for N-point FFTs were also investigated to trade-off between speed and accuracy of the algorithm in view of its application in real-time detection systems.
Time-and-Frequency-domain Analyses of Fluorescence Lifetime for Temperature Sensing / ROSSO L; FERNICOLA V. - In: REVIEW OF SCIENTIFIC INSTRUMENTS. - ISSN 0034-6748. - 77(2006), pp. 034901-1-034901-6.
| Titolo: | Time-and-Frequency-domain Analyses of Fluorescence Lifetime for Temperature Sensing | |
| Autori: | ||
| Data di pubblicazione: | 2006 | |
| Rivista: | ||
| Citazione: | Time-and-Frequency-domain Analyses of Fluorescence Lifetime for Temperature Sensing / ROSSO L; FERNICOLA V. - In: REVIEW OF SCIENTIFIC INSTRUMENTS. - ISSN 0034-6748. - 77(2006), pp. 034901-1-034901-6. | |
| Abstract: | Fluorescence lifetime detection is widely used for sensing physical and chemical quantities. The measurement accuracy of fluorescence lifetime-based sensing systems, either in time or frequency domain relies on their capability of detection and analysis of low level signal superimposed to noise. In this work a quantitative assessment of several data processing and analysis methods for the estimation of the fluorescence lifetime was carried out by using an experimental arrangement based on a fiber optic temperature sensor system. A comparison between the various methods was performed using actual signals from an optical sensing medium. The basic principles of time- and frequency-domain lifetime measurements were also reviewed and discussed in order to point out the limit of the cw frequency-domain approach and to suggest a way to overcome it. The investigated lifetime interval was from 200 to about 2200 s, corresponding to a temperature span of the sensor of about 300 °C. The results showed that in time domain such as with Marquardt, integration, and log-fit algorithms a good agreement, with relative differences from 0.2% to 0.5%, can be reached. Frequency-domain results based on an N-point fast Fourier transform FFT compare favorably with the previous ones in the long lifetime region resulting in relative differences lower than 0.2% with larger differences for short lifetimes. For each data processing method, the uncertainty associated with lifetime estimation was evaluated. Sampling and harmonics effects on the estimation accuracy for N-point FFTs were also investigated to trade-off between speed and accuracy of the algorithm in view of its application in real-time detection systems. | |
| Handle: | http://hdl.handle.net/11696/34668 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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