An optofluidic chip based on a multilayered photonic crystal structure is employed for an efficient beaming of the emitted radiation in a fluorescent microarray assay. Upon a near-field coupling of fluorescent emitters to surface modes sustained by the photonic crystal, a significant portion of radiated energy is diffracted out-of-plane towards the collection optics. The improvement in fluorescence collection is experimentally demonstrated in an antibody-antigene immunoassay. As a result, a decrease of the limit of detection for labelled antigenes by a factor of about 40 is demonstrated to occur in a robust and reproducible way, with little or no optical alignment issues. (C) 2015 Elsevier B.V. All rights reserved.
Optofluidic chip for surface wave-based fluorescence sensing / Ricciardi, S; Frascella, F; Angelini, A; Lamberti, A; Munzert, P; Boarino, Luca; Rizzo, R; Tommasi, A; Descrovi, E.. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 215:(2015), pp. 225-230. [10.1016/j.snb.2015.03.063]
Optofluidic chip for surface wave-based fluorescence sensing
Angelini A;BOARINO, LUCA;
2015
Abstract
An optofluidic chip based on a multilayered photonic crystal structure is employed for an efficient beaming of the emitted radiation in a fluorescent microarray assay. Upon a near-field coupling of fluorescent emitters to surface modes sustained by the photonic crystal, a significant portion of radiated energy is diffracted out-of-plane towards the collection optics. The improvement in fluorescence collection is experimentally demonstrated in an antibody-antigene immunoassay. As a result, a decrease of the limit of detection for labelled antigenes by a factor of about 40 is demonstrated to occur in a robust and reproducible way, with little or no optical alignment issues. (C) 2015 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
