An optofluidic chip based on a multilayered photonic crystal structure is employed for an efficient beaming of the emitted radiation in a fluorescent microarray assay. Upon a near-field coupling of fluorescent emitters to surface modes sustained by the photonic crystal, a significant portion of radiated energy is diffracted out-of-plane towards the collection optics. The improvement in fluorescence collection is experimentally demonstrated in an antibody-antigene immunoassay. As a result, a decrease of the limit of detection for labelled antigenes by a factor of about 40 is demonstrated to occur in a robust and reproducible way, with little or no optical alignment issues. (C) 2015 Elsevier B.V. All rights reserved.

Optofluidic chip for surface wave-based fluorescence sensing / Ricciardi S; Frascella F; Angelini A; Lamberti A; Munzert P; Boarino L; Rizzo R; Tommasi A; Descrovi E. - In: SENSORS AND ACTUATORS. B, CHEMICAL. - ISSN 0925-4005. - 215(2015), pp. 225-230. [10.1016/j.snb.2015.03.063]

Optofluidic chip for surface wave-based fluorescence sensing

Angelini A;BOARINO, LUCA;
2015

Abstract

An optofluidic chip based on a multilayered photonic crystal structure is employed for an efficient beaming of the emitted radiation in a fluorescent microarray assay. Upon a near-field coupling of fluorescent emitters to surface modes sustained by the photonic crystal, a significant portion of radiated energy is diffracted out-of-plane towards the collection optics. The improvement in fluorescence collection is experimentally demonstrated in an antibody-antigene immunoassay. As a result, a decrease of the limit of detection for labelled antigenes by a factor of about 40 is demonstrated to occur in a robust and reproducible way, with little or no optical alignment issues. (C) 2015 Elsevier B.V. All rights reserved.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11696/31492
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