The validation of the AFM method for elastic modulus E measurement in soft materials (E <5 MPa) is still missing. The interest of measurements in materials with E <5 MPa is mainly biological, including soft tissues and single cells. For the diagnosis of malignant human tumors, a change in cell elasticity, within tissues, has recently been recognized as a marker of metastatic potential. To measure a cell elasticity difference, reproducible E measurements in biological samples are needed. In this work a robust method for a metrological validation of E measurements in the range 500–5000 kPa was developed, based on the realization of thick E standard samples and on the study of the interactions between the measurement process and the sample at micro- and nano-scale. E measurement reproducibility limit of 4% has been reached. This allows designing a very sensitive and reproducible measurement of E in biological samples representing thus a powerful diagnostic tool for cancer detection.

Toward the realization of reproducible AFM measurements of elastic modulus in biological samples / Demichelis, A.; Divieto, C.; Mortati, L.; Pavarelli, S.; Sassi, G.; Sassi, M.. - In: JOURNAL OF BIOMECHANICS. - ISSN 0021-9290. - 48:6(2015), pp. 1099-1104. [10.1016/j.jbiomech.2015.01.023]

Toward the realization of reproducible AFM measurements of elastic modulus in biological samples

C. Divieto;L. Mortati;S. Pavarelli;M. Sassi
2015

Abstract

The validation of the AFM method for elastic modulus E measurement in soft materials (E <5 MPa) is still missing. The interest of measurements in materials with E <5 MPa is mainly biological, including soft tissues and single cells. For the diagnosis of malignant human tumors, a change in cell elasticity, within tissues, has recently been recognized as a marker of metastatic potential. To measure a cell elasticity difference, reproducible E measurements in biological samples are needed. In this work a robust method for a metrological validation of E measurements in the range 500–5000 kPa was developed, based on the realization of thick E standard samples and on the study of the interactions between the measurement process and the sample at micro- and nano-scale. E measurement reproducibility limit of 4% has been reached. This allows designing a very sensitive and reproducible measurement of E in biological samples representing thus a powerful diagnostic tool for cancer detection.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11696/30465
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