The cancer diagnosis relies on morphological examination of human cells or tissue samples: This is considered the 'gold standard' for the diagnosis of malignancy in any organ system. However, diagnosis based on morphological examination can be difficult, recent studies recognized the cell elasticity changes as a marker for cancer detection. A metrological characterized AFM can be used to make reproducible elasticity measurements on biological samples. In this paper a method to accurately measure a cell elasticity change based on measurement of a stable and homogeneous material is proposed. Nanoindenter characteristics (nanoindenter with pyramidal tip and triangular shape) and indentation parameters (0.1 V as approaching point, 5 um/s as indentation speed and indentation load of 0.4 V) were found feasible for all tested samples. A modal cell elastic modulus of 0.5 kPa for lung tumor cell and a limit AFM measurement reproducibility of 4% was obtained.

Cell elasticity change as a biomarker for cancer detection: An AFM metrological approach / Demichelis, A.; Divieto, C.; Mortati, L.; Pavarelli, S.; Sassi, M. P.; Sassi, G. - (2018). ((Intervento presentato al convegno 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015 tenutosi a Lecce, Italy nel July 24-25, 2015 [10.1109/NANOFIM.2015.8425323].

Cell elasticity change as a biomarker for cancer detection: An AFM metrological approach

Divieto, C.;Mortati L.;Pavarelli S.;Sassi M. P.;
2018

Abstract

The cancer diagnosis relies on morphological examination of human cells or tissue samples: This is considered the 'gold standard' for the diagnosis of malignancy in any organ system. However, diagnosis based on morphological examination can be difficult, recent studies recognized the cell elasticity changes as a marker for cancer detection. A metrological characterized AFM can be used to make reproducible elasticity measurements on biological samples. In this paper a method to accurately measure a cell elasticity change based on measurement of a stable and homogeneous material is proposed. Nanoindenter characteristics (nanoindenter with pyramidal tip and triangular shape) and indentation parameters (0.1 V as approaching point, 5 um/s as indentation speed and indentation load of 0.4 V) were found feasible for all tested samples. A modal cell elastic modulus of 0.5 kPa for lung tumor cell and a limit AFM measurement reproducibility of 4% was obtained.
1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015
July 24-25, 2015
Lecce, Italy
restricted
File in questo prodotto:
File Dimensione Formato  
08425323.pdf

solo utenti autorizzati

Descrizione: Articolo principale
Tipologia: Versione editoriale
Licenza: Non Pubblico - Accesso privato/ristretto
Dimensione 375.96 kB
Formato Adobe PDF
375.96 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11696/59995
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact