Recent studies recognized the cell stiffness changes as a marker for cancer detection. Reliable and reproducible measurements of elastic modulus, mapping different cell regions are the basis for developing a new methodology for cancer detection. To this aim, a metrological characterized AFM microscope has been developed, micro- and nano-indentation of soft materials, used as elastic modulus reference, were done and a robust statistical data analysis has been performed. Contact images and force mapping of A549 living cells allowed 4% measurement relative reproducibility. A modal cell elastic modulus 0.5 kPa, was obtained. A highly spatially resolved stiffness distribution on the overall cell regions has been obtained through data analysis, realizing a powerful tool for cell mechanics analysis.

Preliminary measurements of elasticity properties of lung tumor living cells for cancer detection / Demichelis, A.; Divieto, C.; Mortati, L.; Sassi, M.; Sassi, G.. - (2015), pp. 240-243. (Intervento presentato al convegno 2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA) tenutosi a Turin nel 7-9 May 2015) [10.1109/MeMeA.2015.7145206].

Preliminary measurements of elasticity properties of lung tumor living cells for cancer detection

C. Divieto;L. Mortati;M. Sassi;
2015

Abstract

Recent studies recognized the cell stiffness changes as a marker for cancer detection. Reliable and reproducible measurements of elastic modulus, mapping different cell regions are the basis for developing a new methodology for cancer detection. To this aim, a metrological characterized AFM microscope has been developed, micro- and nano-indentation of soft materials, used as elastic modulus reference, were done and a robust statistical data analysis has been performed. Contact images and force mapping of A549 living cells allowed 4% measurement relative reproducibility. A modal cell elastic modulus 0.5 kPa, was obtained. A highly spatially resolved stiffness distribution on the overall cell regions has been obtained through data analysis, realizing a powerful tool for cell mechanics analysis.
2015
2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA)
7-9 May 2015
Turin
partially_open
File in questo prodotto:
File Dimensione Formato  
07145206.pdf

non disponibili

Tipologia: final published article (publisher’s version)
Licenza: Non Pubblico - Accesso privato/ristretto
Dimensione 1.21 MB
Formato Adobe PDF
1.21 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
PrePrint-Demichelis Divieto AFM_cells_MeMea 2015.pdf

accesso aperto

Tipologia: submitted version (author’s pre-print)
Licenza: Pubblico - Tutti i diritti riservati
Dimensione 526.3 kB
Formato Adobe PDF
526.3 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11696/52601
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact