Fabry–Perot-based Fourier-transform hyperspectral imaging allows multi-labeled fluorescence analysis

We demonstrate the ability of our hyperspectral imaging device, based on a scanning Fabry–Perot interferometer, to obtain a single hyper-image of a sample marked with different fluorescent molecules, and to unambiguously discriminate them by observing their spectral fingerprints. An experiment carried out with cyanines, fluorescein, and quantum dots emitting in the yellow–orange region, demonstrates the feasibility of multi-labeled fluorescence microscopy without the use of multiple filter sets or dispersive means.